Validation of NS-Forest v2.0 MTG marker genes. (A) t-SNE plots generated using the full 5574 variable gene list, the 157 NS-Forest v2.0 markers, and 157 genes randomly selected from the variable gene list painted by taxonomic assignment. (B) t-SNE map generated from the full 5574 variable gene list was painted by CIELAB color space using coordinate position for each nucleus (left). t-SNE map generated using the 157 NS-Forest markers was then painted according to the CIELAB color space established in the complete variable genes t-SNE (right).