Lineage-specific and parallel (shared) pseudogenization events on the neo-sex chromosomes in Drosophila miranda (mir) and D. albomicans (alb) lineages. A total of 814 orthologs regarded to be functional in the outgroup species (D. obscura and D. kohkoa, respectively) and located on Muller element C without any inparalogs was used for the analysis. Each Venn diagram shows the number of lineage-specific or parallel pseudogenization events on the neo-X (A), neo-Y (B), and ancestral lineages (C; i.e., branches a, b, and c, respectively, in Fig. 4A,C) of D. miranda and D. albomicans. Statistical difference between the observed and expected numbers of shared pseudogenizations was tested by a binomial test with correction for multiple testing: (**) Q < 0.01; (n.s.) Q ≥ 0.05.