TF scCapture-seq observations are corroborated by immunostaining and cell physiology. (A,B) A subpopulation of iPSC-derived cortical neurons were immunopositive for the inhibitory interneuron marker, GAD2, and represented a small subset of DAPI-positive nuclei. (C) Population data showing proportion of GAD2-positive cells (n = 12 fields of view [FOVs] from three differentiations). (D) iPSC-derived cortical neurons were targeted for whole-cell patch clamp recordings. (E) A subset of neurons showed spontaneous outward synaptic currents, consistent with the presynaptic release of GABA from interneurons (top), which could be blocked with the GABA A receptor antagonist, picrotoxin (100 μM; bottom). (F) Population data showing the proportion of neurons receiving GABAergic synaptic input (n = 14).