In-depth characterization of SBS_AⁿT in the whole-genome data from tumor 62074759. (A) SBS spectrum. (B) SBS sequence context preferences, revealing strong preference for adenines 3 bp 3′ of mutated thymines. Thymine mutations predominantly occurred in AAWWTW motifs. (C) Per-mutation view of sequence context preferences of mutations from thymines. Each row represents one mutation, with bases indicated by color as in panel B. (D) Transcriptional strand bias as a function of gene expression level.