csRNA-seq captures the initiation of transient transcripts rapidly degraded by the exosome. (A) Comparison of csRNA-seq with nuclear RNA-seq from wild-type and exosome-depleted HCT116 cells at the ERRFI1 locus (Chr 1: 8,008,489–8,051,491). (B) Global comparison of csRNA-seq with data from the nascent RNA-seq method PRO-seq, as well as wild-type and exosome-depleted nuclear RNA-seq and chromatin profiling (DNase, H3K27ac, H3K4me3) in HCT116 cells.