Distributions of the de novo probabilities in validation and discovery sets in the four cohorts. The y-axis is the predicted de novo probability by Famdenovo.TP53. The width of each column changes over the y-axis, representing the percent of individuals who have the corresponding range of probability values. The individuals with points above the gray line (cutoff = 0.2) were predicted to be DNM carriers. The x-axis gives the names of the cohorts and the corresponding predicted numbers of DNM carriers out of the total number of individuals. (A) In the validation set, the individuals with blue dots were true DNM carriers, and those with red dots were true FM carriers. (B) In the discovery set, the individuals with solid gray dots were predicted to be DNM carriers, and those with empty dots were predicted to be FM carriers.