Mutational consequences of XPC^KO in human intestinal organoid cultures in vitro. (A) Targeting strategy for the generation of XPC^KO organoid cultures using CRISPR-Cas9 gene-editing. (B) Western blot analysis of XPC in human XPC^WT and XPC^KO organoids. (C) Number of base substitutions, (D) double base substitutions, and (E) Signature 8 mutations acquired per autosomal genome per week in human XPC^WT ASCs (n = 3) and an XPC^KO ASC (n = 1) in vitro.