Full-lengths of set2Δ iTSS-derived transcripts use canonical polyadenylation sites. (A) The TSS and TTS comparison between set2Δ iTSSs-initiated transcripts and annotated ORF-T boundaries (Xu et al. 2009). set2Δ iTSS-derived transcripts originate within the body of the gene (internal 5′) but use canonical 3′ polyadenylations sites. (B) Down-regulated TSSs in set2Δ use canonical 5′ and 3′ sites. (C) Example of TIFSeq coverage for the YOL022C gene as an example. The upper part shows TSS mapping (as in Fig. 1A). In the bottom part, we show full-length transcript in blue. Each line connecting between one identified TSS and poly(A) site represents one full-length transcript. The red arrow indicates the appearance of a set2Δ-sensitive iTSS. Nucleosomes are shown in green (Venters and Pugh 2009).