Inference of tumor clones and clonal phylogeny using SiCloneFit for metastatic colorectal cancer patient CRC2. (A) Maximum clade credibility tree reconstructed from the posterior samples obtained using SiCloneFit. Each tumor clone is a cluster of single cells, and their genotypes are also inferred. The temporal order of the mutations is reconstructed, and mutations are annotated on the branches of the clonal tree. The cancer genes and tumor-suppressor genes are marked in purple. The colors of the shades represent the organ/anatomical site of the origin of the cells. (B) Parsimonious migration history of the tumor clones inferred using MACHINA (El-Kebir et al. 2018) with the SiCloneFit inferred clonal tree as input. The top figure shows the clonal tree where the leaves are annotated by the anatomical sites and the anatomical sites annotation of the internal nodes and root are inferred by MACHINA. The bottom figure shows the migration graph of the cells with migration number 2 and comigration number 1. This indicates polyclonal single-source seeding from colon to liver.