Conservation analysis of lncRNA PASs. (A) Three types of lncRNAs and their PASs. (B) Frequency of APA in lncRNAs. (C) Conservation rate of lncRNA PASs. (D) Nucleotide frequency around the PASs of different lncRNAs and mRNAs. (E) Difference in adenosine frequency between lncRNAs and mRNAs in four regions around the PAS: (***) P < 0.001; (**) P < 0.01; (n.s.) not significant (binomial test). (F) Comparison of enriched tetramers between conserved and nonconserved PASs in mRNA genes (left) and lncRNA genes (right). Only the −40 to −1 nt region data are shown. “AAAA” motif is highlighted by an arrow.