bigSCale analysis of 26,185 iCells (convoluted from 1,306,127 single cells) of the embryonic pallium (E18). (A) Dendrogram of 16 iCell clusters representing the major cell types (split by color) and subpopulations (cluster 1–16). Single-cell expression plots (UMI counts) present marker genes (decreasing levels of hierarchical markers) for the main subpopulations and specific markers for neuronal differentiation (lower panel). (B) t-SNE representation of the 16 populations of pallial cells identified by bigSCale clustering. (C) In situ hybridization data for Tubb3 and Slc1a3. Post-mitotic neurons (Tubb3 positive) locate to the outer neocortical layers, including cortical plate (CP) and marginal zone (MR), and radial glia and progenitors (Scl1a3 positive) are found in the ventricular and subventricular zone (VZ).