Assessment of the cell convolution strategy in bigSCale. (A) Comparison of original and convoluted clustering with the Rand index (RI). Pairwise cell comparisons were performed for three increasing degrees of convolution (Conv1,2,3) into iCells (numbers indicated). Similarity of clustering (RI; y-axis) were evaluated at different resolution (n cluster numbers; x-axis). RI were >80% for all tested combinations, pointing to highly similar cluster assignment for original and iCells. (B) t-SNE plots comparing original and convoluted clustering. The example displays a comparison with RI = 82% and 12 clusters. The high degree of concordance between experiments is visible through the consistent cluster assignment of cell pairs.