ESAG composition of selected telomere clones per BES type. Representative telomere clones (indicated in the third column) were analyzed by PCR using three primer sets per ESAG to minimize problems associated with analyzing polymorphic gene families with the exception of ESAG10 and ESAG11, in which two primer sets were used (for ESAG primers used, see Table 1). Results are indicated with + if all ESAG primer sets gave a positive result. If a subset of the primer sets gave positive results, these are indicated with a superscript number (1 indicates first primer set; 2, second primer set; 3, third primer set) (see Table 1). If the result was inconclusive, that is, after three repetitions of the PCR, mixed positive, negative, or weak positive results were obtained, this is indicated with m. Negative PCR results for all three primer sets are indicated with –. If the TAR clones analyzed for a given BES set differed, a comma separates the two results.
