Analysis of SPANX-D and SPANX-B coding sequences in X-linked families. The alignment of SPANX-D (A) and SPANX-B (B) coding sequences obtained from prostate cancer patients (top), healthy controls (middle), and genomic SPANX-A/D genes (bottom). The figure shows the results of analysis of 10 families, some of them are represented by two brothers (shown by brackets). When two SPANX-B copies were present in the same individual, they were marked by _B1 and _B2 suffices. IUPAC codes of the variants, synonymous (S) and nonsynonymous (N) characters of changes, and positions in the CDS alignment are shown at the bottom; positions highlighted by boxes correspond to CpG sites and their TpG/CpA variants. If several mutations occurred in the same codon, they were marked by a dash. In all such cases, the resulting effect was an amino acid replacement, and therefore, all such changes were marked as nonsynonymous.