ptr-4(RNAi) disrupts endocytosis. (A–C) A YP170::GFP reporter is expressed in the intestine and endocytosed by the proximal-most oocyte (arrowheads) in wild-type hermaphrodites, as visualized by GFP fluorescence in C. (D–F) ptr-4(RNAi) hermaphrodites inefficiently localize YP170::GFP to oocytes (arrowheads). Panels A, B, D, and E are Nomarski DIC micrographs; panels C, F show YP170::GFP fluorescence. Scale bar, 50 μm.