Gene shuffling flanking a thymidylate synthase locus. The ORFs represented among FN and FNV genomes are colored and numbered as a set according to homology and paired bidirectional best hits. The sets are (1) thymidylate synthase, (2) hypothetical protein, (3) hypothetical protein, (4) copper-exporting ATPase, (5) COP associated protein, (6) poly(A) polymerase/tRNA nucleotidyltransferase, (7) potassium uptake protein, (8) dihydrofolate reductase, (9) hypothetical protein, (10) thiamine-binding protein, (11) thiamine transport system, and (12) hypothetical protein. Set 1 contains ORFs FNV01239 and FN01321.