Activation of MAGE family by the Spi-B transcription factor. (A) MAGE cluster identified by SP analysis. (B) Up-regulation of MAGE genes by Spi-B. The 293 cells were transiently transfected with either an empty vector (con) or a vector expressing Spi-B (Spi). At 48-hour post-transfection, cells were harvested and RNAs prepared for real-time PCR analysis. Results are the average of two experiments, each performed in duplicate.