Structure and expression of chicken PHEMX/TSSC6. (A) RT-PCR analysis of chicken PHEMX/TSSC6 in whole chick embryos and adult tissues. GAPD was used as a housekeeping control. Splicing variants, revealed by sequencing of the PCR products, are shown (isoforms 1–6). (B) Nucleotide sequence and predicted amino acid sequence of chicken PHEMX/TSSC6 cDNA (isoform 1; GenBank accession no. AB101639). Arrowheads indicate the positions of exon–intron boundaries. (C) Alignment of the predicted amino acid sequences of human (GenBank accession no. AF125569), mouse (GenBank accession no. AJ279791), and chicken PHEMX/TSSC6. Amino acids conserved in more than two species are shaded.