Overview of promoterome cloning procedure. (A) Electrophoretic analysis of the genomic PCR products. The 8929 PCR reactions were analyzed on 1% agarose E-Gels. Ninety-five percent (95%) of the reactions gave visible PCR products of the expected size (primer pairs were arranged so that for each row the product size increases from 300 to 2000 bp). (B) Schematic representation of large-scale cloning of the C. elegans promoterome. All cloning steps are performed in a 96-well format. Clones of the expected size are consolidated using a Tecan Gemini robot, and stored both as miniprep DNA and bacterial glycerol stock.