Figure 4

Photomicrographs of GFP expression patterns for previously uncharacterized genes. (A) Hypodermal and pharyngeal expression for C06G4.4 in a late larva of strain UL1171. (B) Pharyngeal nerve cell expression for bas-1 / C05D2.4 in strain UL1164. (C) Expression in the spermatheca for C26E6.4 in a young adult of strain UL1173 presented with fluorescent and Nomarski images. (D) The general expression for C16A3.4 in UL1162 includes the developing vulva. (E) Nucleolar-localized expression in intestinal nuclei of strain UL1166 for C06E1.10. In WormBase (www.wormbase.org), C06E1.10 has been identified as an ATP-dependent RNA helicase, which would be consistent with the nucleolar localization. (F) Three different focal planes through the head of a young adult from strain UL1167 reveals discreet subcellular GFP localization, in subcellular foci and apparently at specific cellular surfaces, in different cell types, for C02F5.9. In WormBase (www.wormbase.org), C02F5.9 has been identified as a proteasome β-subunit.

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