Pipeline for selection of ORFeome clones and summary of results from RNAi by soaking. (A) ORFeome v. 1.1 contains 11,875 clones. Of these, 1582 represent ovary-enriched genes. We chose the 1245 clones from this set representing genes for which no embryonic requirement had been established by RNAi by any study and which we had not tested previously. From these we tested 1123 clones and found 241 that showed a high-confidence Emb or Ste phenotype. After removing clones for which sequence could not be verified and mapping the clones to gene models from WormBase (WS110), we identified 195 unique genes (excluding alternative splice forms) with high-confidence Emb or Ste phenotypes, 155 of which are new and ovary-enriched. (B) We compared phenotypes for the 26 genes we tested that were also represented by characterized mutant alleles. For 18 of these, both RNAi and genetic analysis revealed an Emb or Ste phenotype. Seven were identified only by genetics, and one was identified only by RNAi.