C20orf16 gene locus. This figure shows the transcript predicted from the cDNA pieces on the C20orf16 locus in chromosome 20. The aligned cDNAs are given in blue; the cDNA pieces, in green; and the predictions, in red. The three original cDNAs are correctly recovered from the chunks. However, one extra chimeric combination of chunks is produced: the uppermost of the predictions. This happens when the cDNA pieces do not cover two or more neighboring sites of alternative splicing.