Sensitivity (Sn) and specificity (Sp) for 5′ UTR predictions in the human genome by comparison to aligned sequences from DBTSS. All Sp values are scaled assuming 25,000 genes in human genome and assuming the annotation set is a random sample of the full set of human genes. (A) TSS predictions for Eponine (tan), DGSF (light yellow), FirstEF (light blue), and N-SCAN (red). (B) First exon predictions for FirstEF (light blue) and N-SCAN (red) and from FirstEF evaluated in 15-kb windows upstream of annotated start codons (light purple).