PS1010 is an outgroup for C. elegans and its close relatives. (A) Percent identity values for the FOG-1 and FOG-3 proteins of each species. (B) Neighbor-joining tree of nematode FOG-1 sequences. Because they were hard to align, we excuded the amino terminus of each FOG-1 protein from this analysis; these excluded sequences totaled 60–128 amino acid residues per protein. (The total size range for the FOG-1 proteins was 564–653 residues). We chose the Oscheius tipulae CEW1 CPEB-A protein as our outgroup, and used PAUP* 4.0b10 for the calculations. Bootstrap confidence values are presented at each node, and were based on 10,000 replications.