Map of int1h and int22h duplicons. (Top) F8 gene (clear box), with arrow indicating direction of transcription, plus sequence of int1h (black boxes) and int22h (gray boxes) with arrows indicating orientation. Clear and solid arrow heads indicate location and orientation of primers flanking int22h copies and int1h copies, respectively (for primer sequences see Liu et al. 1998 and Bagnall et al. 2002). (Bottom) Int22h and int1h showing location and orientation of primers listed in Supplemental Table 3. These were primers required for completing the sequence of the chimpanzee's int22h sequences (solid arrow heads), or for allele-specific PCR in int22h (clear arrow heads), or for amplification of DNA for FSPCCM analysis (lines terminating with arrow heads). Other primers for sequencing int22h were from Naylor et al. (1995) (not shown). Primers for sequencing int1h (solid arrow heads and underlined names) are from Bagnall et al. (2002).