HSAT I semi-quantitative PCR on hominoid DNA. HSAT I, beta-catenin (CTNNB1), and SMARCB1 (viral integrase 1; two control primer sets) were used to amplify human, chimpanzee, pygmy, bonobo, gorilla, and orangutan DNA and then were electrophoresed in a 1.5% agarose gel. The PCR was done with increasing cycles 20, 25, and 30. (Lanes 1–7) HSAT I PCR, (lanes 8–14) CTNNB1, (lanes 15–21) SMARCB1. The lanes are human–chimpanzee–pygmy–bonobo–gorilla–orangutan–negative control for each PCR primer and each cycling condition. The control primers do not amplify at the low cycles very well. In the HSAT I PCR, human (1) and gorilla (5) have an increased amplification compared to the other species.