Analysis of size distribution of cloned fragments and comparison to agarose gel image. Candida albicans genomic DNA was sheared to an apparent size range of 2.5–5 kb and separated by agarose gel electrophoresis. (A) The gel image was digitized as described in Fig. 5 and imported into StatView 4.5. After shearing, the DNA was ligated into pUC118 to form a library. Eighty-four inserts from the library were measured by PCR amplification and analysis of product size on an agarose gel. (B) A frequency distribution of the insert size. The dark line shows the normal curve that was calculated as the best fit for the data. The shaded line shows a densitometer trace of the electrophoretogram in A. The data in B are plotted such that the tick marks on the x-axis line up approximately with the DNA size markers in A.