Centromere repositioning in C. albicans. PCR analysis of CENPA/Cse4 and CENPC1/Mif2 ChIP DNA in wild-type RM1000AH (upper), RM1000AH-cen7-6.5kbΔ (middle) harboring nCEN7-III (∼13 kb away from URA3 and marked by a star), and a gene-converted derivative of RM1000AH-cen7-6.5kbΔ (bottom). Absence of CENPA and CENPC1 binding at the nCEN7-III locus after gene conversion confirms neocentromere inactivation.