HSAT I and HSAT II FISH mapping. (A) FISH mapping on metaphase human male peripheral lymphocytes stained with DAPI with HSAT II probe (green) and HSAT I (red) have an overlapping signal producing a yellow color. (B) Fiber-FISH on chromosome Y with HSAT I probe (red) and HSAT II (green), show more hybridization of HSAT II on the Yq12 region compared with HSAT I. There is no distinct pattern of hybridization of HSAT I and HSAT II; however, HSAT II hybridizes to a larger region of the Yq12 region. (C) An enlarged region of one of the DNA fiber strands from B.