Florescence intensity for autosomal (diploid) loci. VDA features are tiled with 25mers. The number of As, Cs, Gs, and Ts were counted for the reference 25mer on both the forward and reverse strand. The florescence at each pixel within the feature was measured (>3.9 billion pixels in total). Error bars represent two standard errors, under the assumption that separate features are independent but pixels within a feature are not.