CRAW output (Java version) for a UniGene101 cluster with a cancer-specific alternative gene form. Subgroup 1 (in green) ESTs are identical to CAMK. The green regions of subgroup 2 are identical to subgroup 1 sequences, and the blue region of subgroup 2 diverges. This is an example of how effective subparitioning can add sensitivity to gene expression specificity studies. If one were to seek cancer-specific genes by looking at the original UniGene index cluster, the level of specificity to cancer libraries would only be ∼53% = (100 × 9/17)%; however, at the subcluster level, the specificity level is 100%.