Regulatory interactions between classic and novel regulators of neural crest development. Functional studies reveal epistatic interactions between neural plate border and neural crest specifiers with CNC genes. (A) Summary of the results obtained from the loss-of-function experiments. (B–F) Representative embryos for each of the phenotype classes: (B) no phenotype; (C) mild knockdown; (D) strong knockdown; (E) mild up-regulation; and (F) strong up-regulation. (G) Quantification of knockdown experiments.