PUF60 and RBM10 regulate endogenous exon 18 splicing in cultured primary neurons. (A) Steady-state mRNA levels in primary neurons after transfection of a pair of Accell siRNAs targeting either the Puf60 or Rbm10 transcript. (B) The Puf60 and Rbm10 siRNAs alter endogenous exon 18 splicing in primary neurons at DIV 5. Exon 18 PSI values are plotted and a representative gel is shown. Values represent mean ± SD (N = 3). (*) P < 0.05 (Student’s t-test, in comparison to the control [Ctr]).