HIPK2 and MED19 depletion selectively affects the proliferation of AR-expressing prostate cancer cells. AR-positive prostate cancer cell lines (A) LNCaP and (B) LNCaP-abl and AR-negative (C) HEK293 and (D) PC3 cells were transfected with control siRNA (siControl), HIPK2 siRNA (siHIPK2), or MED19 siRNA (siMED19), and cell proliferation was measured at the indicated days and shown as relative fluorescence units (RFUs). (E–H) The efficiency of knockdown was monitored at the mRNA level for each factor in the four cell lines relative to RLP19. Each experiment was repeated at least two times. A representative experiment is shown.