The EWS–FLI complex is capable of altering chromatin. (A) Normalized signals for H3K4me1 (black), H3K4me2 (red), H3K4me3 (blue), H3K27me3 (green), and FAIRE (orange) from both EWS502 and HUVEC are plotted for the 2-kb region surrounding the summits of sites identified by hierarchical clustering. (B) Change in FAIRE enrichment at EWS–FLI-bound GGAA repeats following EWS–FLI expression in HUVEC. All values are represented as fold-change relative to scrambled shRNA control. Error bars represent the standard error from three technical replicates. (C) Change in FAIRE enrichment at EWS–FLI-bound GGAA repeats following EWS–FLI silencing in EWS502. All values are represented as fold change relative to scrambled shRNA control. Error bars represent the standard error from three technical replicates.