Developmentally regulated genetic profile for genes associated with loss-of-fitness in all three experiments involving the bloodstream form (BF) and compared to the equivalent procyclic form (PF) profile. (A) Genes in the BF (0-0-1-0, red bars) and PF (1-1-0-0, gray bars) groups are presented as a Z-score heat-map above the associated GO-term profile. All highly significant (P < 0.01) associations from the GO-slim set are shown. See Supplemental File 2 for full GO-term analysis. Every term shown is notably under-represented in the PF group. Terms shown in blue are analyzed in more detail in B and discussed in the text. (B) Mean Z-scores for cohorts of genes associated with flagellar motility and the proteasome complex. (IFT) Intraflagellar transport. (C) Mean Z-scores for cohorts of genes associated with energy metabolism. (PDH) Pyruvate dehydrogenase. (B–C) See Supplemental File 1B for details of cohorts of genes analyzed.