Comparison of BandLeader (A) and IMAGE (B) performance on test gels. “Real fragment size”, plotted on thex-axes, refers to restriction fragment size as determined by computer analysis of “finished” human and mouse BAC sequence data. Fragment sizes determined by BandLeader or IMAGE are plotted on they-axes. Fragments less than 600 bp are not considered. Each data point represents the comparison of a restriction fragment predicted by sequence analysis to a restriction fragment identified by either IMAGE or BandLeader. Red lines on the plot indicate a 2% size window. Points falling within the 2% window are considered to represent identical restriction fragments. “Total called” refers to the number of restriction fragments identified by IMAGE or BandLeader. “Total correct” refers to the number of these fragments that match the corresponding sequence-predicted fragments. This is a measure of specificity. “Total real” refers to the number of sequence-predicted fragments, and “total found” refers to the number of these detected by the software. This is a measure of sensitivity.