Sequence alignment of three different cases between EST and the rat WGS reads. (A) DNA alignment of region conserved in human, mouse and rat. The GenBank entry number for the human EST is AW139193 and the mouse cDNA is AK0055990. (B) Protein alignment of region conserved in human, mouse, and rat. In addition, the putative protein is likely to be conserved in Drosophila and inCaenorhabditis elegans. The letter z in the human sequence indicates a stop codon. The fact that the mouse and rat sequences continue to be very similar to each other before and after this stop codon indicates some sequencing or cloning error in the human sequence. The GenBank entry number for the human EST is BE784449, the mouse cDNA is AK013451, the fly is AAF45939.1, and the C. elegans isAAB53055.1. (C) DNA alignment of region that is only observed in human and rat; no mouse EST has been found. The human EST GenBank accession number is BF028817.