RNAi-mediated depletion of selected hits in N1E cells validates their effect on the splicing of endogenous Dlg4 exon 18. (A) Steady-state mRNA levels of the tested genes were significantly decreased in N1E cells at 72 h after transfection of the target-specific siRNAs. Two different siRNAs targeting each gene were examined. After the knockdown with each siRNA, endogenous exon 18 splicing was assayed by RT-PCR (B). The PSI of exon 18 was plotted for each siRNA, and a representative gel is shown. The dashed line marks the basal level of exon 18 inclusion. Values represent mean ± SD (N = 3). (*) P < 0.05 (Student’s t-test, in comparison to the control [Ctr]).