ChIP-seq analyses show that CEN proximal neocentromeres are authentic neocentromeres in Chr1 and Chr5. Relative number of sequence reads from whole cell lysate (top panel) or CENPA-Prot A ChIP DNA (lower panels) in J200 (wild type), J227 (RM1000AH-cen1Δ), J225, or J226 (RM1000AH-cen5Δ) mapped neocentromeres (nCENs) within 15 kb from the deleted region. (Left panels) Distribution of sequence reads across the entire chromosome; (right panels) zoomed-in view of the neocentromeric location along with the native CEN in each case. Chromosome coordinates are shown in the x-axis.