ChIP analysis of H3K4me2, H3K4me2, and H3K4me3 chromatin in transgene cassettes in RL5 cells. (A) RL5 cells with a transgene cassette carrying a 9-kb gamma-satellite DNA. (B) RL5 cells with a transgene cassette carrying a 10-kb alphoid DNA. Position of the primers for cassettes with gamma-satellite (A) and alpha-satellite (B) DNAs are as follows: (1) centromeric side of RL5 locus; (2) YAC vector backbone; (3) BAC vector backbone; (4) gamma- or alpha-satellite arrays; (5) 5′ end of the locus control region; (6) eGFP coding region; and (7) telomeric side of RL5 locus. Sequences of primers for two control loci, the murine beta-major globin locus and the murine amylase locus, are presented in Supplemental Table S10. (C) RL5 cells with p212 cassette. Positions of the primers are as follows: (1) centromeric side of RL5 locus; (5) 5′ end of the locus control region; (6) eGFP coding region; and (7) telomeric side of RL5 locus. (D) RL5 cells with pYB cassette. Positions of the primers are as follows: (1) centromeric side of RL5 locus; (2) YAC vector backbone; (3) BAC vector backbone; (5) 5′ end of the locus control region; (6) eGFP coding region; and (7) telomeric side of RL5 locus.