Distribution of nucleosomes and anti-sense TSS around 3′ end of genes. (A) Distribution of nucleosome locations relative to the 3′ end of open reading frames (ORFs). Nucleosome distances relative to the ORF stop codon were binned in 10 bp intervals, normalized to the number of regions represented in each bin (red line), then smoothed using a three-bin moving average. Regions internal to adjacent genes or within 360 bp of another ORF start site (whichever is closer) were either removed (black fill) or not removed (gray fill) from the analysis. The distribution of polyA sites, where transcripts terminate (TTS), is shown as a blue trace. (B) Distribution of anti-sense TSS distances from 3′ NFRs. Anti-sense TSS coordinates were from (Perocchi et al. 2007). Distances were binned (10 bp bin) and smoothed (three-bin moving average).