Simultaneous analysis of 16 different samples in four capillary columns containing samples that were homozygous or heterozygous for exon 13, 22, or 26 in MDR1. Columns, 4 × monolithic PS/DVB, 60 × 0.2 mm i.d.; mobile phase, (A) 100 mM TEAA at pH 7.0, (B) 100 mM TEAA at pH 7.0, 25% acetonitrile; linear gradient, 45%–70% B in 10.0 min; flow rate, 2.0–3.0 μL/min; temperature, (a,d) 55°C, (b) 56°C, (c) 57°C; detection, LIF, emission monitored at 525 nm for FAM (blue), 555 nm for HEX (green), 580 nm for NED (yellow), and 605 nm for ROX (red); injection volume, 1 μL each; samples, column 1, exon 22, FAM, homozygote, exon 13, HEX, 52 T>G and 291 T>C double heterozygote, exon 26, NED, 212 C>T and 346 T>C double heterozygote, exon 26, ROX, homozygote; column 2, exon 22, FAM, 320 G>A heterozygote, exon 13, HEX, homozygote, exon 26, NED, homozygote, exon 26, ROX, 212 C>T and 346 T>C double heterozygote; column 3, exon 22, FAM, homozygote, exon 13, HEX, homozygote, exon 13, NED, 52 T>G and 291 T>C double heterozygote, exon 26, ROX, homozygote; column 4, exon 22, FAM, homozygote, exon 13, HEX, 52 T>G and 291 T>C double heterozygote, exon 13, NED, homozygote, exon 26, ROX, homozygote.