PCR-based screening of BAC DNA pools for SAS-DNA markers using AFLP technology. AFLP templates were prepared from BAC DNA PPs (1–32) and SPs (1–16) and selectively amplified with fluorescent-labeledEcoRI + TGA and MseI + CGG. Labeled products were analyzed on a LI-COR DNA sequencer. AFLP template from genomic DNAs (IS3620C and BTx623) were run as controls and are indicated above the respective lanes. Arrows to the right of the gel show selected SAS DNA markers that were analyzed in the DNA pools. Asterisks to the right of a subset of the markers indicate those SAS DNAs that revealed polymorphisms between BTx623 and IS3620C and could be mapped as AFLPs on the sorghum genetic map. Fluorescent-labeled molecular weight markers (LI-COR) were run in lanes marked M and their sizes (bp) are shown to the left of the gel.