Amplified cDNA from total RNA from the S. pneumoniae strain R6x grown in C+Y supplemented with 0.2% raffinose and 0.2% glucose. In each reaction (lanes 1–10) one primer pair was used, as indicated by the lines. The expected size for each fragment is given in kb. Lane 11 is a negative control (−) without RT and the primer pair from lane 3 in the reaction mixture; Lane12 is a positive control (+) with total RNA and primers provided with the Access RT–PCR introductory System. The arrows indicate the identified potential promoters.