HIPK2 depletion affects AR target gene expression. HIPK2 knockdown decreases the expression of AR target genes in LNCaP (A) and LNCaP-abl (B) cells. Cells were transfected with control (siControl) or HIPK2 siRNA (siHIPK2) and either androgen deprived for 48 h and then treated with 10 nM R1881 for 24 h (LNCaP) or cultured in media with 10% charcoal-stripped FBS for 48 h (LNCaP-abl). Relative mRNA levels of the indicated genes were analyzed by qPCR and normalized to RPL19. Each assay was performed in duplicate, with error bars representing the range of the mean.