Copy-number variation spanning MAPK-locus on chromosome 36. Copy number per cell in L. donovani (red, SSG-R; blue, SSG-S) was much higher for the MAPK locus than the chromosome 36 median for L. donovani (black dashed line) and L. infantum (black). The amplified region is marked in gray and their white edges indicate the location of two direct repeats. Genes within and adjacent to the amplified region are given in black and light gray, respectively. The MAPK (mitogen-activated protein kinase) gene is labeled as LMPK (LdBPK_366760). Recombination events may occur through a highly homologous 220-bp region starting at the start codon of the tartrate-sensitive acid phosphatase (LdBPK_366740) and histidine secretory acid phosphatase (LdBPK_366770). LdBPK_366750 encodes a protein of unknown function. Two RIME (L. donovani ribosomal mobile elements; light-blue) flank the duplicated region. Read-depth values were computed using 1-kb sliding windows, and error bars are shown for each kilobase.