Nuclear position of chromosome 17 territories and fosmids covering differentially expressed genes in normal and t(11;22) cells. The fosmid coordinates and mapping positions are shown in Table 1 and Supplemental Figure S6, respectively. (A) Mean (+SEM) percent of chromosome 17 paint hybridization signal present in five shells of equal area eroded from the edge (shell 1) to the center (shell 5) of the nucleus in four control cell lines of normal karyotype (dark purple) and five t(11;22) cell lines (light purple) showing the relative positions of the chromosome 17s. n = 40–50 nuclei each for each cell lines. The chromosome 17 territory is shifted to a more peripheral position in nuclei from balanced translocation carriers as compared to controls. (B) Mean (+SEM) percent of the hybridization signal of chromosome 9q21.32 fosmid G248P81402D5 (D5) in four control cell lines of normal karyotype (dark blue) and four t(11;22) cell lines (pink) showing the relative positions of the fosmid. The fosmid signal is shifted to a slightly more peripheral position in nuclei from balanced cell lines as compared with controls. (C) As in B, but using fosmid G248P8942D11 (D11) on three control cell lines of normal karyotype (right) and four t(11;22) cell lines (left), showing the relative positions of the fosmid on the normal 11 (blue) and on the derivative chromosome 11 (green). The fosmid signal on the derivative 11 is shifted to a slightly more peripheral position than that on the normal 11. (D) As in B, but using fosmid G248P84992F11 (F11) on three control cell lines (left) and four t(11;22) cell lines (right), showing the relative positions of the normal 22 (red) and the derivative 11 (green). The fosmid signal on the derivative 11 is shifted to a slightly more central position than that on the normal 22. (E) As in B, but using fosmid G248P87917B7 (B7) on four control cell lines (left) and five t(11;22) cell lines (right), showing the relative positions of the normal 22 (red) and the derivative 11 (green). This fosmid covers a gene that does not show any differential expression between balanced translocation carriers and controls and the fosmid signal on the derivative 11 shows no difference in position compared with that on the normal 22.