Transcriptional activity of the transferred Wolbachia genes. (A) RT-PCR assay of 57 transferred Wolbachia genes. The circle is identical to the inner circle of Figure 1. Open dots indicate genes with amplified products, while filled dots show genes without amplification. (B) Quantitative RT-PCR assay of 35 transferred Wolbachia genes, of which 26 were RT-PCR positive and nine were RT-PCR negative. An exon region and an intron region of the insect ef1α gene were also quantified as positive and negative controls. Each of the green diamonds shows an RNA sample from an insect treated with reverse transcriptase, while each of the red dots indicates an RNA sample from an insect without reverse transcriptase. In the ORF line, open dots indicate structurally intact ORFs, while filled dots show disrupted ORFs. In the RT-PCR line, open dots and filled dots indicate RT-PCR–positive and RT-PCR–negative, respectively.