LIX1 multiple tissue Northern blot. Twenty micrograms of total RNA isolated from each normal cat tissue was electrophoresed on a 0.8% formaldehyde-agarose gel, transferred to a nylon membrane, and hybridized to a random-primed labeled LIX1 cDNA probe that included 280 bp of 5′ UTR, the coding region, and 545 bp of 3′ UTR. Migration of RNA molecular weight markers (in kb) is indicated to the left. (Lanes: 1, thalamus; 2, spinal cord ventral horn; 3, kidney; 4, cardiac muscle; 5, skeletal muscle).